Abstract The emergence of Roller Skates infectious diseases worldwide necessitates rapid and precise diagnostics.Using gold nanoshells in the PCR mix, we harnessed their unique photothermal properties in the near-infrared regime to attain efficient heating, reaching ideal photothermal PCR cycle temperature profile.Our photothermal PCR method expedited DNA amplification while retaining its detection sensitivity.Combining photothermal quantitative PCR with real-time fluorometry and non-invasive temperature measurement, we could amplify the target DNA within just 25 min, with a minimum detectable DNA amount of 50 picograms.This innovation in photothermal qPCR, leveraging the photothermal Accessories properties of gold nanoshells, will pave the way for immediate point-of-care diagnostics of nucleic acid biomarkers.